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ID 96861
Author
Nishimura, Yumiko Dental Department, Hiroshima University Hospital
Oyama, Yasuo Graduate School of Integrated Arts and Sciences, The University of Tokushima KAKEN Search Researchers
Keywords
Lidocaine
Intracellular Zn2+ level
FluoZin-3
Content Type
Departmental Bulletin Paper
Description
Lidocaine augmented FluoZin-3 fluorescence of rat thymocytes, suggesting the increase in intracellular Zn2+
concentration. However, the mechanism for lidocaine-induced increase in the intensity of FluoZin-3
fluorescence was not elucidated. In this study, in order to reveal the mechanism, the effects of lidocaine on
FluoZin-3 and 5-chloromethylfluorescein (5-CMF) fluorescence were examined under various conditions.
5-CMF fluorescence was used as a parameter for cellular content of nonprotein thiols. The lidocaine-induced
augmentation of FluoZin-3 fluorescence was observed under external Ca2+- and/or Zn2+-free conditions,
suggesting that lidocaine induced intracellular Zn2+ release. Lidocaine attenuated 5-CMF fluorescence,
suggesting the decrease in cellular content of nonprotein thiols. Since some peptides consisting of SH-group
have been proposed to store Zn2+, the decrease in nonprotein thiol content by
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Volume
26
Issue
2
Start Page
7
End Page
10
Sort Key
7
Published Date
2012-04
EDB ID
FullText File
language
jpn
departments
Bioscience and Bioindustry