ID 96861
Author
Nishimura, Yumiko Dental Department, Hiroshima University Hospital
Oyama, Yasuo Graduate School of Integrated Arts and Sciences, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
Lidocaine
Intracellular Zn2+ level
FluoZin-3
Content Type
Departmental Bulletin Paper
Description
Lidocaine augmented FluoZin-3 fluorescence of rat thymocytes, suggesting the increase in intracellular Zn2+
concentration. However, the mechanism for lidocaine-induced increase in the intensity of FluoZin-3
fluorescence was not elucidated. In this study, in order to reveal the mechanism, the effects of lidocaine on
FluoZin-3 and 5-chloromethylfluorescein (5-CMF) fluorescence were examined under various conditions.
5-CMF fluorescence was used as a parameter for cellular content of nonprotein thiols. The lidocaine-induced
augmentation of FluoZin-3 fluorescence was observed under external Ca2+- and/or Zn2+-free conditions,
suggesting that lidocaine induced intracellular Zn2+ release. Lidocaine attenuated 5-CMF fluorescence,
suggesting the decrease in cellular content of nonprotein thiols. Since some peptides consisting of SH-group
have been proposed to store Zn2+, the decrease in nonprotein thiol content by
Journal Title
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
NCID
AN10065859
Volume
26
Issue
2
Start Page
7
End Page
10
Sort Key
7
Published Date
2012-04
EDB ID
FullText File
language
jpn
departments
Bioscience and Bioindustry