ID 109641
Author
Iwata, Takeo Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School KAKEN Search Researchers
Kuwajima, Masamichi Department of Clinical Biology and Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School
Sukeno, Akiko Department of Clinical Biology and Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School
Ishimaru, Naozumi Department of Oral Molecular Pathology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Hayashi, Yoshio Department of Oral Molecular Pathology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Wabitsch, Martin Division of Pediatric Endocrinology, Department of Pediatrics and Adolescent Medicine, University of Ulm
Mizusawa, Noriko Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Itakura, Mitsuo Division of Genetic Information, Institute for Genome Research, The University of Tokushima Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Yoshimoto, Katsuhiko Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Keywords
YKL-40
MMP-1
type I collagen
adipose tissue
macrophage
preadipocyte
Content Type
Journal Article
Description
Obesity is considered a chronic low-grade inflammatory status and the stromal vascular fraction (SVF) cells of adipose tissue (AT) are considered a source of inflammation-related molecules. We identified YKL-40 as a major protein secreted from SVF cells in human visceral AT. YKL-40 expression levels in SVF cells from visceral AT were higher than in those from subcutaneous AT. Immunofluorescence staining revealed that YKL-40 was exclusively expressed in macrophages among SVF cells. YKL-40 purified from SVF cells inhibited the degradation of type I collagen, a major extracellular matrix of AT, by matrix metalloproteinase (MMP)-1 and increased rate of fibril formation of type I collagen. The expression of MMP-1 in preadipocytes and macrophages was enhanced by interaction between these cells. These results suggest that macrophage/preadipocyte interaction enhances degradation of type I collagen in AT, meanwhile, YKL-40 secreted from macrophages infiltrating into AT inhibits the type I collagen degradation.
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006291X
NCID
AA00564395
Volume
388
Issue
3
Start Page
511
End Page
516
Sort Key
511
Published Date
2009-10-23
Remark
© 2009 Elsevier Inc. All rights reserved.
© 2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
Published Source
Biochemical and Biophysical Research Communications (2009) Volume 388 Issue 3 P.511–516 (doi:10.1016/j.bbrc.2009.08.024)
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Oral Sciences
Institute of Advanced Medical Sciences