ID 111463
Title Alternative
TRH AND SRIF IN MICRODIALYSATES
Author
Mizobuchi, Miki The University of Tokushima
Ishikawa, Masayasu The University of Tokushima
Okauchi, Yasuhiro The University of Tokushima
Bando, Hiroshi The University of Tokushima KAKEN Search Researchers
Saito, Shiro The University of Tokushima
Keywords
Thyroidectomy
TRH
Somatotropin release-inhibiting factor (SRIF)
Microdialysis
Adenohypophysis
Central feedback action
Content Type
Journal Article
Description
The effects of thyroidectomy on patterns of TRH and somatotropin release-inhibiting factor (SRIF) release from the hypothalamus were investigated by using a microdialysis technique. Thyroidectomized and sham-operated rats underwent placement of a guide cannula into the anterior pituitary gland to obtain dialysates, or implantation of an intravenous cannula into the right atrium for blood sampling. Seven days postoperatively dialysates were collected at a flow rate of 2μl/min every 1 h. TRH concentrations in dialysates from thyroidectomized rats (0.43±0.22pg/h) were significantly higher than those from control rats (0.17±0.02pg/h). In contrast, SRIF concentrations in dialysates from thyroidectomized rats (2.45±0.05pg/h) were significantly lower than those from control rats (3.80 ±0.22pg/h). In addition, plasma TSH concentrations in thyroidectomized rats (24.8±0.5ng/ml) were increased compared with those in control rats (2.5±0.1ng/ml), and plasma GH concentrations were decreased from 68.6±6.4ng/ml in control rats to 21.2±0.6ng/ml in thyroidectomized rats. These findings indicate that TRH and SRIF releases from the hypothalamus are detectable by microdialysis method, and directly show the increase in TRH secretion and the decrease in SRIF secretion from hypothalamus in the hypothyroid state.
Journal Title
Endocrine Journal
ISSN
13484540
09188959
NCID
AA10901436
Publisher
The Japan Endocrine Society
Volume
43
Issue
6
Start Page
679
End Page
687
Published Date
1996
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Publisher
departments
Oral Sciences