ID 111463
タイトル別表記
TRH AND SRIF IN MICRODIALYSATES
著者
Mizobuchi, Miki The University of Tokushima
Ishikawa, Masayasu The University of Tokushima
Okauchi, Yasuhiro The University of Tokushima
板東, 浩 The University of Tokushima KAKEN研究者をさがす
Saito, Shiro The University of Tokushima
キーワード
Thyroidectomy
TRH
Somatotropin release-inhibiting factor (SRIF)
Microdialysis
Adenohypophysis
Central feedback action
資料タイプ
学術雑誌論文
抄録
The effects of thyroidectomy on patterns of TRH and somatotropin release-inhibiting factor (SRIF) release from the hypothalamus were investigated by using a microdialysis technique. Thyroidectomized and sham-operated rats underwent placement of a guide cannula into the anterior pituitary gland to obtain dialysates, or implantation of an intravenous cannula into the right atrium for blood sampling. Seven days postoperatively dialysates were collected at a flow rate of 2μl/min every 1 h. TRH concentrations in dialysates from thyroidectomized rats (0.43±0.22pg/h) were significantly higher than those from control rats (0.17±0.02pg/h). In contrast, SRIF concentrations in dialysates from thyroidectomized rats (2.45±0.05pg/h) were significantly lower than those from control rats (3.80 ±0.22pg/h). In addition, plasma TSH concentrations in thyroidectomized rats (24.8±0.5ng/ml) were increased compared with those in control rats (2.5±0.1ng/ml), and plasma GH concentrations were decreased from 68.6±6.4ng/ml in control rats to 21.2±0.6ng/ml in thyroidectomized rats. These findings indicate that TRH and SRIF releases from the hypothalamus are detectable by microdialysis method, and directly show the increase in TRH secretion and the decrease in SRIF secretion from hypothalamus in the hypothyroid state.
掲載誌名
Endocrine Journal
ISSN
13484540
09188959
cat書誌ID
AA10901436
出版者
The Japan Endocrine Society
43
6
開始ページ
679
終了ページ
687
発行日
1996
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系