ID | 111925 |
Title Alternative | N-Sulfanylethylanilide-based traceable linker for enrichment and selective labelling of target proteins
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Author |
Morisaki, Takuya
Tokushima University
Denda, Masaya
Tokushima University
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Yamamoto, Jun
Tokushima University
Inokuma, Tsubasa
Tokushima University
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Itoh, Kohji
Tokushima University
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Shigenaga, Akira
Tokushima University|Japan Science and Technology Agency (JST)
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Otaka, Akira
Tokushima University
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Content Type |
Journal Article
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Description | An N-sulfanylethylanilide-based traceable linker, developed to facilitate identification of target proteins of bioactive compounds, was introduced into an alkynylated target protein. Subsequent adsorption onto streptavidin beads allowed it to be treated with a cysteine-fluorophore conjugate in the presence of phosphate. This induced the N-S acyl transfer reaction of the N-sulfanylethylanilide unit. The subsequent native chemical ligation of the fluorophore resulted in cleavage of the linker for target elution and fluorescent labelling of the target, allowing it to be distinguished from non-target proteins.
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Journal Title |
Chemical Communications
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ISSN | 13597345
1364548X
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NCID | AA11071130
AA12455105
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Publisher | The Royal Society of Chemistry
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Volume | 52
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Issue | 42
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Start Page | 6911
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End Page | 6913
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Published Date | 2016-04-26
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Rights | ©The Royal Society of Chemistry 2016
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EDB ID | |
DOI (Published Version) | |
URL ( Publisher's Version ) | |
FullText File | |
language |
eng
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TextVersion |
Author
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departments |
Pharmaceutical Sciences
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