ID 111925
Title Alternative
N-Sulfanylethylanilide-based traceable linker for enrichment and selective labelling of target proteins
Author
Morisaki, Takuya Tokushima University
Denda, Masaya Tokushima University
Yamamoto, Jun Tokushima University
Shigenaga, Akira Tokushima University|Japan Science and Technology Agency (JST) Tokushima University Educator and Researcher Directory KAKEN Search Researchers
Content Type
Journal Article
Description
An N-sulfanylethylanilide-based traceable linker, developed to facilitate identification of target proteins of bioactive compounds, was introduced into an alkynylated target protein. Subsequent adsorption onto streptavidin beads allowed it to be treated with a cysteine-fluorophore conjugate in the presence of phosphate. This induced the N-S acyl transfer reaction of the N-sulfanylethylanilide unit. The subsequent native chemical ligation of the fluorophore resulted in cleavage of the linker for target elution and fluorescent labelling of the target, allowing it to be distinguished from non-target proteins.
Journal Title
Chemical Communications
ISSN
13597345
1364548X
NCID
AA11071130
AA12455105
Publisher
The Royal Society of Chemistry
Volume
52
Issue
42
Start Page
6911
End Page
6913
Published Date
2016-04-26
Rights
©The Royal Society of Chemistry 2016
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Pharmaceutical Sciences