ID | 111925 |
タイトル別表記 | N-Sulfanylethylanilide-based traceable linker for enrichment and selective labelling of target proteins
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著者 |
Morisaki, Takuya
Tokushima University
Yamamoto, Jun
Tokushima University
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資料タイプ |
学術雑誌論文
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抄録 | An N-sulfanylethylanilide-based traceable linker, developed to facilitate identification of target proteins of bioactive compounds, was introduced into an alkynylated target protein. Subsequent adsorption onto streptavidin beads allowed it to be treated with a cysteine-fluorophore conjugate in the presence of phosphate. This induced the N-S acyl transfer reaction of the N-sulfanylethylanilide unit. The subsequent native chemical ligation of the fluorophore resulted in cleavage of the linker for target elution and fluorescent labelling of the target, allowing it to be distinguished from non-target proteins.
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掲載誌名 |
Chemical Communications
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ISSN | 13597345
1364548X
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cat書誌ID | AA11071130
AA12455105
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出版者 | The Royal Society of Chemistry
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巻 | 52
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号 | 42
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開始ページ | 6911
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終了ページ | 6913
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発行日 | 2016-04-26
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権利情報 | ©The Royal Society of Chemistry 2016
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
著者版
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部局 |
薬学系
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