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ID 111925
タイトル別表記
N-Sulfanylethylanilide-based traceable linker for enrichment and selective labelling of target proteins
著者
Morisaki, Takuya Tokushima University
Denda, Masaya Tokushima University
Yamamoto, Jun Tokushima University
重永, 章 Tokushima University|Japan Science and Technology Agency (JST) 徳島大学 教育研究者総覧 KAKEN研究者をさがす
資料タイプ
学術雑誌論文
抄録
An N-sulfanylethylanilide-based traceable linker, developed to facilitate identification of target proteins of bioactive compounds, was introduced into an alkynylated target protein. Subsequent adsorption onto streptavidin beads allowed it to be treated with a cysteine-fluorophore conjugate in the presence of phosphate. This induced the N-S acyl transfer reaction of the N-sulfanylethylanilide unit. The subsequent native chemical ligation of the fluorophore resulted in cleavage of the linker for target elution and fluorescent labelling of the target, allowing it to be distinguished from non-target proteins.
掲載誌名
Chemical Communications
ISSN
13597345
1364548X
cat書誌ID
AA11071130
AA12455105
出版者
The Royal Society of Chemistry
52
42
開始ページ
6911
終了ページ
6913
発行日
2016-04-26
権利情報
©The Royal Society of Chemistry 2016
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
薬学系