ID 113348
Author
Komiya, Chiaki Tokushima University
Tsukimoto, Jun Tokushima University
Ueda, Masahiro Tokushima University
Morisaki, Takuya Tokushima University
Content Type
Journal Article
Description
A traceless thioester-producing protocol featuring carboxypeptidase Y-mediated hydrazinolysis of cysteinyl prolyl leucine-tagged peptides has been developed. The hydrazinolysis followed by thioesterification affords cysteinyl prolyl thioesters. Self-editing of the tag and subsequent trans-thioesterification yields peptide thioesters. The developed protocol was successfully applied to conversion of recombinant proteins to thioesters.
Journal Title
Chemical Communications
ISSN
13597345
1364548X
NCID
AA11071130
AA12455105
Publisher
The Royal Society of Chemistry
Volume
55
Issue
49
Start Page
7029
End Page
7032
Published Date
2019-05-23
Remark
論文本文は2020-05-23以降公開予定
Rights
©The Royal Society of Chemistry 2019
DOI (Published Version)
URL ( Publisher's Version )
language
eng
TextVersion
その他
departments
Pharmaceutical Sciences