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ID 114845
Author
Mine, Masanori Tokushima University
Keywords
Capillary electrophoresis
Alkaline phosphatase
Inhibition assay
Content Type
Journal Article
Description
A novel inhibition assay is proposed by capillary electrophoresis/dynamic frontal analysis (CE/DFA). When a substrate of p-nitrophenyl phosphate and an inhibitor of theophylline were tandemly introduced into the capillary containing alkaline phosphatase as an enzyme, two plateau signals were detected in the electropherogram. A higher plateau is based on the CE/DFA without inhibition, and a suppressed plateau is formed under the inhibition while the substrate zone passing through the inhibitor zone. Inhibition constant was successfully determined through the two plateau heights.
Journal Title
Chemistry Letters
ISSN
13480715
NCID
AA12179675
Publisher
The Chemical Society of Japan
Volume
49
Issue
6
Start Page
681
End Page
684
Published Date
2020-03-26
Rights
© 2020 The Chemical Society of Japan
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Science and Technology