アクセス数 : ?
ダウンロード数 : ?
ID 114845
著者
Mine, Masanori Tokushima University
キーワード
Capillary electrophoresis
Alkaline phosphatase
Inhibition assay
資料タイプ
学術雑誌論文
抄録
A novel inhibition assay is proposed by capillary electrophoresis/dynamic frontal analysis (CE/DFA). When a substrate of p-nitrophenyl phosphate and an inhibitor of theophylline were tandemly introduced into the capillary containing alkaline phosphatase as an enzyme, two plateau signals were detected in the electropherogram. A higher plateau is based on the CE/DFA without inhibition, and a suppressed plateau is formed under the inhibition while the substrate zone passing through the inhibitor zone. Inhibition constant was successfully determined through the two plateau heights.
掲載誌名
Chemistry Letters
ISSN
13480715
cat書誌ID
AA12179675
出版者
The Chemical Society of Japan
49
6
開始ページ
681
終了ページ
684
発行日
2020-03-26
権利情報
© 2020 The Chemical Society of Japan
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
理工学系