ID 110171
著者
水野, 孝彦 Graduate School of Science and Technology, Tokushima University
岩田, 哲郎 Graduate School of Science and Technology, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
資料タイプ
学術雑誌論文
抄録
We discuss a Hadamard-transform-based fluorescence-lifetime-imaging (HT-FLI) technique for fluorescence-lifetime-imaging microscopy (FLIM). The HT-FLI uses a Fourier-transform phase-modulation fluorometer (FT-PMF) for fluorescence-lifetime measurements, where the modulation frequency of the excitation light is swept linearly in frequency from zero to a specific maximum during a fixed duration of time. Thereafter, fluorescence lifetimes are derived through Fourier transforms for the fluorescence and reference waveforms. The FT-PMF enables the analysis of multi-component samples simultaneously. HT imaging uses electronic exchange of HT illumination mask patterns, and a high-speed, high-sensitivity photomultiplier, to eliminate frame-rate issues that accompany two-dimensional image detectors.
掲載誌名
Optics Express
ISSN
10944087
24
8
開始ページ
8202
終了ページ
8213
並び順
8202
発行日
2016-04-07
備考
Copyright © 2016 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.
EDB ID
出典
Optics Express (2016) Vol.24 Issue 8 p.8202-8213
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
理工学系