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ID 110171
著者
水野, 孝彦 Graduate School of Science and Technology, Tokushima University 徳島大学 教育研究者総覧
岩田, 哲郎 Graduate School of Science and Technology, Tokushima University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
資料タイプ
学術雑誌論文
抄録
We discuss a Hadamard-transform-based fluorescence-lifetime-imaging (HT-FLI) technique for fluorescence-lifetime-imaging microscopy (FLIM). The HT-FLI uses a Fourier-transform phase-modulation fluorometer (FT-PMF) for fluorescence-lifetime measurements, where the modulation frequency of the excitation light is swept linearly in frequency from zero to a specific maximum during a fixed duration of time. Thereafter, fluorescence lifetimes are derived through Fourier transforms for the fluorescence and reference waveforms. The FT-PMF enables the analysis of multi-component samples simultaneously. HT imaging uses electronic exchange of HT illumination mask patterns, and a high-speed, high-sensitivity photomultiplier, to eliminate frame-rate issues that accompany two-dimensional image detectors.
掲載誌名
Optics Express
ISSN
10944087
24
8
開始ページ
8202
終了ページ
8213
並び順
8202
発行日
2016-04-07
備考
Copyright © 2016 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.
EDB ID
出典
Optics Express (2016) Vol.24 Issue 8 p.8202-8213
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
理工学系