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ID 113980
著者
Hashimoto, Muneaki National Institute of Advanced Industrial Science and Technology
Yatsushiro, Shouki National Institute of Advanced Industrial Science and Technology
Yamamura, Shohei National Institute of Advanced Industrial Science and Technology
Tanaka, Masato National Institute of Advanced Industrial Science and Technology
Sakamoto, Hirokazu National Institute of Advanced Industrial Science and Technology|The University of Tokyo
井戸, 佑介 National Institute of Advanced Industrial Science and Technology 徳島大学 教育研究者総覧
Kajimoto, Kazuaki National Institute of Advanced Industrial Science and Technology
片岡, 正俊 National Institute of Advanced Industrial Science and Technology
キーワード
Malaria
Diagnosis
Automation
Hydrophilic treatment
Giemsa-staining
資料タイプ
学術雑誌論文
抄録
Background: Malaria is a red blood cell (RBC) infection caused by Plasmodium parasites. To determine RBC infection rate, which is essential for malaria study and diagnosis, microscopic evaluation of Giemsa-stained thin blood smears on glass slides (‘Giemsa microscopy’) has been performed as the accepted gold standard for over 100 years. However, only a small area of the blood smear provides a monolayer of RBCs suitable for determination of infection rate, which is one of the major reasons for the low parasite detection rate by Giemsa microscopy. In addition, because Giemsa microscopy is exacting and time-consuming, automated counting of infection rates is highly desirable.
Results: A method that allows for microscopic examination of Giemsa-stained cells spread in a monolayer on almost the whole surface of hydrophilic-treated cyclic olefin copolymer (COC) plates was established. Because wide-range Giemsa microscopy can be performed on a hydrophilic-treated plate, the method may enable more reliable diagnosis of malaria in patients with low parasitaemia burden. Furthermore, the number of RBCs and parasites stained with a fluorescent nuclear staining dye could be counted automatically with a software tool, without Giemsa staining. As a result, researchers studying malaria may calculate the infection rate easily, rapidly, and accurately even in low parasitaemia.
Conclusion: Because the running cost of these methods is very low and they do not involve complicated techniques, the use of hydrophilic COC plates may contribute to improved and more accurate diagnosis and research of malaria.
掲載誌名
Malaria Journal
ISSN
14752875
出版者
BioMed Central|Springer Nature
16
開始ページ
321
発行日
2017-08-08
権利情報
© The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
先端酵素学研究所
病院
歯学系