ID | 117763 |
著者 |
Ogura, Akihiro
RIKEN
Nozaki, Satoshi
RIKEN
Morimoto, Koji
Osaka Women’s Junior College
Kizuka, Yasuhiko
RIKEN
Kitazume, Shinobu
RIKEN
Hara, Mitsuko
RIKEN
Kojima, Soichi
RIKEN
Onoe, Hirotaka
RIKEN
Kurbangalieva, Almira
Kazan Federal University
Taniguchi, Naoyuki
RIKEN
Watanabe, Yasuyoshi
RIKEN
Tanaka, Katsunori
RIKEN|Kazan Federal University|Japan Science and Technology Agency
|
資料タイプ |
学術雑誌論文
|
抄録 | A series of N-glycans, each sequentially trimmed from biantennary sialoglycans, were homo- or heterogeneously clustered efficiently on fluorescent albumin using a method that combined strain-promoted alkyne-azide cyclization and 6π-azaelectrocyclization. Noninvasive in vivo kinetics and dissection analysis revealed, for the first time, a glycan-dependent shift from urinary to gall bladder excretion mediated by sequential trimming of non-reducing end sialic acids. N-glycoalbumins that were trimmed further, in particular, GlcNAc- and hybrid biantennary-terminated congeners, were selectively taken up by sinusoidal endothelial and stellate cells in the liver, which are critical for diagnosis and treatment of liver fibrillation. Our glycocluster strategy can not only reveal the previously unexplored extracellular functions of N-glycan trimming, but will be classified as the newly emerging glycoprobes for diagnostic and therapeutic applications.
|
掲載誌名 |
Scientific Reports
|
ISSN | 20452322
|
出版者 | Springer Nature
|
巻 | 6
|
開始ページ | 21797
|
発行日 | 2016-02-23
|
権利情報 | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
|
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
|
著者版フラグ |
出版社版
|
部局 |
先端研究推進センター
|