| ID | 82750 |
| 著者 |
谷村, 綾子
Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
KAKEN研究者をさがす
ヤマダ, フミヨ
Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
サイトウ, アキヒト
Department of Applied Molecular Medicine, Niigata University Graduate School of Medicine and Dental Sciences
伊藤, 美紀子
Department of Food Science and Nutrition, School of Human Science and Environment, University of Hyogo
キムラ, トオル
Department of Pharmacology,School of Medicine, Kyorin University
アンザイ, ナオヒコ
Department of Pharmacology,School of Medicine, Kyorin University
ホリエ, ダイスケ
Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
山本, 浩範
Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
KAKEN研究者をさがす
宮本, 賢一
Department of Molecular Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
徳島大学 教育研究者総覧
KAKEN研究者をさがす
竹谷, 豊
Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
徳島大学 教育研究者総覧
KAKEN研究者をさがす
武田, 英二
Department of Clinical Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School
徳島大学 教育研究者総覧
KAKEN研究者をさがす
|
| キーワード | native PAGE
macromolecular complex
NHERF1
PDZK1
megalin
|
| 資料タイプ |
学術雑誌論文
|
| 抄録 | Type IIa sodium-dependent phosphate transporter (NaPi-IIa) can be localized
in the apical plasma membrane of renal proximal tubule to carry out a rate-limiting step of phosphate reabsorption. For the apical localization, NaPi-IIa is required to form a macromolecular complex with some adaptor proteins such as Na+/H+ exchanger regulatory factor 1 (NHERF-1) and ezrin. However, the detail of macromolecular complex containing NaPi-IIa in the apical membrane of the renal proximal tubular cells has not been clarified. In this study, we identified at least four different complexes (220, 480, 920, 1,100 kDa) containing NaPi-IIa by using blue-native polyacrylamide gel electrophoresis. Interestingly, LC-MS/MS analysis and immunoprecipitation analysis reveal that megalin is a component of larger complexs (920 and 1,100 kDa). In addition, NaPi-IIa can be heterogeneously co-localized with ezrin and megalin on the apical membrane of renal proximal tubuler cells by fluorescence microscopy analysis. These results suggest that NaPi-IIa can form some different complexes on the apical plasma membrane of renal proximal tubular cells. |
| 掲載誌名 |
The journal of medical investigation : JMI
|
| ISSN | 13431420
|
| cat書誌ID | AA11166929
|
| 巻 | 58
|
| 号 | 1-2
|
| 開始ページ | 140
|
| 終了ページ | 147
|
| 並び順 | 140
|
| 発行日 | 2011-02
|
| 備考 | The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
|
| EDB ID | |
| フルテキストファイル | |
| 言語 |
eng
|
| 部局 |
歯学系
医学系
|
