| ID | 114845 |
| 著者 |
Mine, Masanori
Tokushima University
|
| キーワード | Capillary electrophoresis
Alkaline phosphatase
Inhibition assay
|
| 資料タイプ |
学術雑誌論文
|
| 抄録 | A novel inhibition assay is proposed by capillary electrophoresis/dynamic frontal analysis (CE/DFA). When a substrate of p-nitrophenyl phosphate and an inhibitor of theophylline were tandemly introduced into the capillary containing alkaline phosphatase as an enzyme, two plateau signals were detected in the electropherogram. A higher plateau is based on the CE/DFA without inhibition, and a suppressed plateau is formed under the inhibition while the substrate zone passing through the inhibitor zone. Inhibition constant was successfully determined through the two plateau heights.
|
| 掲載誌名 |
Chemistry Letters
|
| ISSN | 13480715
|
| cat書誌ID | AA12179675
|
| 出版者 | The Chemical Society of Japan
|
| 巻 | 49
|
| 号 | 6
|
| 開始ページ | 681
|
| 終了ページ | 684
|
| 発行日 | 2020-03-26
|
| 権利情報 | © 2020 The Chemical Society of Japan
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| EDB ID | |
| 出版社版DOI | |
| 出版社版URL | |
| フルテキストファイル | |
| 言語 |
eng
|
| 著者版フラグ |
著者版
|
| 部局 |
理工学系
|
