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ID 117138
タイトル別表記
Novel method for detection of pancreatic beta cell death using cell-free DNA
著者
岡田, 朝美 徳島大学
山田, 美鈴 徳島大学
明比, 祐子 徳島大学
キーワード
Insulin
DNA methylation
RT-PCR
Type1 diabetes
cell-free DNA
資料タイプ
学術雑誌論文
抄録
In people with type1 diabetes(T1D), biomarkers that can sensitively and quantitatively evaluate injury of pancreatic beta cell are required in order to predict the onset of the disease at an early stage and to provide interventions to prevent the progression of the disease. We developed a new method for quantifying pancreatic beta cell-derived insulin DNA in circulation that combines bisulfite conversion and Amplification Refractory Mutation System(ARMS)PCR, which can be performed using a conventional real-time PCR system. We applied this method to T1D patients and healthy adults, both could be detected in about 30% of cases. The results in healthy adults indicate that this method may have sensitivity to detect the turnover of pancreatic beta cells at physiological conditions. In post-onset T1D patients, there were many negatives because the amount of residual pancreatic beta cells was extremely small. However, in some cases with a short duration of the disease, pancreatic beta cell-derived insulin DNA was detected in negative correlation between the duration of the disease, that suggested the residual pancreatic beta cells continue to be slowly destroyed. It was demonstrated that the time course of pathophysiology in T1D could be understood using this method.
掲載誌名
四国医学雑誌
ISSN
00373699
cat書誌ID
AN00102041
出版者
徳島医学会
77
5-6
開始ページ
249
終了ページ
254
並び順
249
発行日
2021-12-25
EDB ID
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
先端酵素学研究所
病院
医学系