直近一年間の累計
アクセス数 : ?
ダウンロード数 : ?
ID 116383
タイトル別表記
片側末梢投与されたA型ボツリヌス毒素は動物モデルにおいて両側三叉神経節に局在する
著者
ワスキソ, アリーフ 徳島大学大学院口腔科学教育部(口腔科学専攻)
Yamamoto, Yumiko Okayama University
Raman, Swarnalakshmi Tokushima University
Yan, Huijiao Tokushima University
Raju, Resmi National Center for Geriatrics and Gerontology
Afroz, Shaista Aligarh Muslim University
キーワード
Botulinum toxin
trigeminal ganglion
neuropathic pain
資料タイプ
学位論文
抄録
Peripheral nerve injury leads to sensory ganglion hyperexcitation, which increases neurotransmitter release and neuropathic pain. Botulinum toxin type A (BoNT/A) regulates pain transmission by reducing neurotransmitter release, thereby attenuating neuropathic pain. Despite multiple studies on the use of BoNT/A for managing neuropathic pain in the orofacial region, its exact mechanism of transport remains unclear. In this study, we investigated the effects of BoNT/A in managing neuropathic pain in two different animal models and its transport mechanism in the trigeminal nerve. Intraperitoneal administration of cisplatin induced bilateral neuropathic pain in the orofacial region, reducing the head withdrawal threshold to mechanical stimulation. Unilateral infraorbital nerve constriction (IONC) also reduced the ipsilateral head withdrawal threshold to mechanical stimulation. Unilateral peripheral administration of BoNT/A to the rat whisker pad attenuated cisplatin-induced pain behavior bilaterally. Furthermore, contralateral peripheral administration of BoNT/A attenuated neuropathy-induced behavior caused by IONC. We also noted the presence of BoNT/A in the blood using the mouse bioassay. In addition, the Alexa Fluor-488-labeled C-terminal half of the heavy chain of BoNT/A (BoNT/A-Hc) was localized in the neurons of the bilateral trigeminal ganglia following its unilateral administration. These findings suggest that axonal and hematogenous transport are involved in the therapeutic effects of peripherally administered BoNT/A in the orofacial region.
掲載誌名
Toxins
ISSN
20726651
出版者
MDPI
13
10
開始ページ
704
発行日
2021-10-05
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Arief Waskithoの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3609号
学位記番号
甲口第477号
学位授与年月日
2022-03-23
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系
病院