ID 110758
著者
Bunpo, Piyawan Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School|Department of Biochemistry, Faculty of Medicine, Chiang Mai University
片岡, 佳子 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
有持, 秀喜 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
中山, 治之 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
桑原, 知巳 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School
Vinitketkumnuen, Usanee Department of Biochemistry, Faculty of Medicine, Chiang Mai University
大西, 克成 Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School 徳島大学 教育研究者総覧
キーワード
asiatic acid
apoptosis
CPT-11
combination
cytotoxicity
資料タイプ
学術雑誌論文
抄録
Asiatic acid is a pentacyclic triterpene contained inmedicinal plants. The cytotoxic effect of this compound and its augmentative effect on the anticancer drug irinotecan hydrochloride (CPT-11) were investigated in the human colon adenocarcinoma cell lineHT-29. Asiatic acid dose-dependently showed cytotoxicity in HT-29 cells. DNA fragmentation, annexin-positive apoptotic cells, andcaspase-3 activation were observed in a dose-dependent manner. Acaspase-3 inhibitor suppressed the DNA ladder formation in a concentration-dependent manner. Bcl-2 and Bcl-xL proteins were decreased by asiatic acid treatment. These results indicate that asiatic acid induced apoptosis inHT-29 cells viacaspase-3activation.Cytotoxic effectsof combined treatment with CPT-11 and asiatic acid on HT-29 cells were further examined. Simultaneous treatment or sequential exposure first to asiatic acid and then to CPT-11 showed an additive effect. Synergism was observed when cells were first exposed to CPT-11 and then to asiatic acid. These results suggest that asiatic acid can be used as an agent for increasing sensitivity of colon cancer cells to treatment with CPT-11 or as an agent for reducing adverse effects of CPT-11.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
52
1-2
開始ページ
65
終了ページ
73
並び順
65
発行日
2005-02
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系