キムラ, カオリ Faculty of Dentistry, The University of Tokushima
ニシムラ, ユミコ Faculty of Dentistry, Okayama University
オヤマ, ケイスケ Faculty of Medicine, Saga University
カワナイ, タクヤ Faculty of Integrated Arts and Sciences, The University of Tokushima
ハシモト, エリカ Faculty of Integrated Arts and Sciences, The University of Tokushima
In this study to examine the cytotoxic property of tetracaine, we cytometrically examined the effect of tetracaine on intracellular Zn2+ concentration by the use of FluoZin-3, a fluorescent indicator of intracellular Zn2+. Lidocaine was used as a reference drug. The incubation of rat thymocytes with tetracaine decreased the intensity of FluoZin-3 fluorescence while that with lidocaine increased the intensity. The incubation with 10 μM DTPA, a chelator for extracellular Zn2+, attenuated the tetracaine-induced decrease in fluorescence intensity. The application of ZnCl2 augmented FluoZin-3 fluorescence. The augmentation by ZnCl2 was a temperature-sensitive. Tetracaine attenuated the ZnCl2-induced augmentation of FluoZin-3 fluorescence. Taken together, the results suggest that tetracaine attenuates membrane Zn2+ influx, resulting in a decrease in intracellular Zn2+ concentration in rat thymocytes. Although the cells in this study are not targets for actions of local anesthetics, the result may give one clue to explain the difference between the cytotoxicity of local anesthetics since the action of tetracaine on FluoZin-3 fluorescence was opposite to that of lidocaine.
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
LID201104261001.pdf 218 KB