性分化と精子形成機構に対するプロテオミクスからのアプローチ

SRY （sex determining region on the Y chromosome） was a gene isolated as the testis determining factor on the Y chromosome in １９９０．The gene consisted of single exon and encodes a ２０４ amino acid protein. Since SRY protein has a HMG domain, which functions as DNA binding domain, it has been considered to be a transcription factor. Direct target of SRY, however, have not yet identified for １４ years.
In this study, we have performed a proteomics approach to analyze the function of SRY. Twodimensional gel electrophoresis after over expression of SRY showed considerable down regulation in many proteins. Among the proteins, mitochondrial ６０‐kDa heat shock protein （HSP６０） and probable protein disulfide isomerase （ER６０） remarkably decreased. Since they were reported to be associated with cell proliferation and differentiation, we investigated the effects of SRY on cell cycle profiles. It was shown that the over‐expression of SRY negatively regulated the cell growth with significant S or G２／M arrest of cell cycle.

四国医学雑誌 : http://www.tokushima-u.ac.jp/med/research/shikoku_acta_medica.html