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ID 116063
タイトル別表記
血管内皮細胞における過酸化水素誘導のカベオリン-1リン酸化に対するケルセチンの抑制効果
著者
河井(近藤), あかり 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
キーワード
quercetin
caveolae
caveolin-1
hydrogen peroxide
vascular endothelium
資料タイプ
学位論文
抄録
Caveolin-1 is a major protein of the caveolae structure in vascular endothelial cell membrane. Phosphorylation of caveolin-1 is one of the initial events leading to exacerbation of vascular permeability caused by oxidative stress. Although quercetin is known to be an anti-atherosclerosis factor that acts as a dietary antioxidant, little is known about its role in the regulation of caveolin-1 phosphorylation. In this study, we investigated the inhibitory effect of quercetin on hydrogen peroxide-induced caveolin-1 phosphorylation in human umbilical vein endothelial cells. Quercetin inhibited caveolin-1 phosphorylation in cells pretreated with quercetin for 24 h and then exposed to hydrogen peroxide. However, quercetin 3-O-β-glucuronide, a conjugated metabolite of quercetin, did not exert this inhibitory effect. Exposure to hydrogen peroxide increased vascular permeability and reduced mRNA expression of the intercellular adhesion protein, vascular endothelial cadherin (VE-cadherin). By contrast, pretreatment with quercetin suppressed the increase in vascular permeability and decreased VE-cadherin expression. These results indicate that deconjugated quercetin can play a role in the prevention of altered vascular permeability under oxidative stress by suppressing caveolin-1 phosphorylation. Thus, dietary quercetin may be beneficial for the maintenance of endothelial cell function.
掲載誌名
Journal of Clinical Biochemistry and Nutrition
ISSN
09120009
18805086
cat書誌ID
AA10710201
出版者
Society for Free Radical Research Japan
69
1
開始ページ
28
終了ページ
36
発行日
2021-07-01
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Akari Kondo-Kawaiの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
© 2020 Society for Free Radical Research Japan
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License. https://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3510号
学位記番号
甲栄第284号
学位授与年月日
2021-03-23
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系
生物資源系