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ID 114473
著者
Li, Wei Tokyo Institute of Technology
Michinobu, Tsuyoshi Tokyo Institute of Technology
Sakata, Toshiya The University of Tokyo
キーワード
Catecholamine
Dopamine
Molecularly imprinted polymer
Field-effect transistor
Surface-initiated atom transfer radical polymerization
資料タイプ
学術雑誌論文
抄録
We report a well-designed biointerface enabling the selective and quantitative detection of dopamine (DA) using a potentiometric biosensor. To enhance the detection selectivity of DA, a DA-templated molecularly imprinted polymer (DA–MIP) was synthesized on the extended Au gate electrode of a field-effect transistor (FET) biosensor. For a quantitative DA analysis, the thickness of the DA–MIP was controlled to ca. 60 nm by surface-initiated atom transfer radical polymerization (SI-ATRP). In this process, the DA–MIP was copolymerized with vinylphenylboronic acid (vinyl-PBA), inducing molecular charges at the biointerface of the FET gate electrode. These charges were generated by the diol-binding between PBA and dopamine (a catecholamine), and were directly detected as a change in surface potential. In fact, the surface potential at the gate of the DA–MIP-coated FET responded significantly to DA added at concentrations ranging from 40 nM to μM, whereas that of a non-imprinted polymer (NIP)-coated FET hardly changed over this range. Moreover, by measuring the kinetic parameters and electrochemical properties of well-designed devices with various added catecholamines, we confirmed that the DA–MIP-coated FET biosensor selectively and quantitatively detects DA.
掲載誌名
Biosensors and Bioelectronics
ISSN
09565663
cat書誌ID
AA10739666
AA1152282X
出版者
Elsevier
117
開始ページ
810
終了ページ
817
発行日
2018-07-11
権利情報
© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
ポストLEDフォトニクス研究所