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ID 113896
著者
Okuda, Tomoyo Kyoto University
Kikukawa, Hiroshi Gifu University
Ando, Akinori Kyoto University
Kishino, Shigenobu Kyoto University
Izumi, Yoshihiro Kyushu University
Bamba, Takeshi Kyushu University
Shima, Jun Ryukoku University
Ogawa, Jun Kyoto University
キーワード
Mortierella alpina
Oleic acid
Linoleic acid
Fatty acid desaturase
RNAi
資料タイプ
学術雑誌論文
抄録
The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76 g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain.
掲載誌名
Bioresource Technology
ISSN
18732976
09608524
cat書誌ID
AA11522750
AA10838373
出版者
Elsevier
245
B
開始ページ
1610
終了ページ
1615
発行日
2017-06-19
権利情報
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系