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ID 115576
著者
Sugano, Shigeo S. The University of Tokushima
キーワード
CRISPR/Cas9
Genome editing
Genomics
Molecular breeding
Woody plants
資料タイプ
学術雑誌論文
抄録
Engineered endonucleases that digest the specific sequences can be used to modify target genomes precisely. This is called “genome editing” and is used widely to modify the genome of various organisms. The DNA-binding domains of zinc finger (ZF) proteins were the first to be used as a genome editing tool, in the form of designed ZF nucleases and, more recently, transcription activator-like effector as well as the clustered, regularly interspaced, short palindromic repeats and CRISPR-associated protein 9 (CRISPR/Cas9) system that targets RNA–DNA rather than protein–DNA interactions have been used successfully. These are powerful tools with which targeted gene modifications can be introduced in various organisms, including various plant species. A key step in genome editing is the generation of a double-stranded DNA break that is specific to the target gene. This is achieved using custom-designed endonucleases, which enable site-directed mutagenesis via a non-homologous end joining repair pathway and/or gene targeting via homologous recombination to occur efficiently at specific sites in the genome. This review provides an overview of the current status of genomics and genetic engineering of woody plants, and recent advances in genome editing technologies in plants as well as fungi. We also discuss how these strategies can provide insights into molecular breeding technology for woody plants.
掲載誌名
Journal of Wood Science
ISSN
16114663
cat書誌ID
AA11200007
出版者
The Japan Wood Research Society|Springer Nature
62
3
開始ページ
217
終了ページ
225
発行日
2016-03-11
権利情報
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系