ID 111640
著者
Masamune, Katsuhiro The University of Tokushima
Kunitomo, Kazufumi Tezuka Hospital
Sasaki, Kenji The University of Tokushima
Yagi, Keiko The University of Tokushima
Komi, Nobuhiko The University of Tokushima
キーワード
anomalous arrangement of the pancreaticobiliary ducts
bile acid fractions
DNA strand breaks
in situ nick translation
資料タイプ
学術雑誌論文
抄録
A canine experimental model for the anomalous arrangement of the pancreaticobiliary ducts (APBD) was made to investigate the effects of bile acids on carcinogenesis. Seven adult mongrel dogs underwent dorsal pancreatico-cholecystostomy to serve as a functional model for APBD, and six dogs underwent the same procedure with the pancreatic duct ligated as a control group. Bile from the gallbladder was taken 14 months after surgery for bile acid analysis by HPLC. DNA strand breaks in HeLa cells induced by the bile were also investigated in situ by nick translation method. As a result, the fraction of cholic acid tended to be lower, and that of deoxycholic acid slightly higher in APBD-dogs (N.S.). The ursodeoxycholic acid percentage in APBD-dogs significantly decreased compared with that in the control and normal dogs (p<0.05). Extremely high frequency of DNA strand breaks was shown in only two out of seven APBD-dogs. In those two dogs, the cholic acid percentage decreased and that of deoxycholic acid increased extremely. These findings suggest that the alteration of the bile composition in APBD caused frequent DNA strand breaks and repair which might lead to gene mutation and biliary tract carcinoma.
掲載誌名
The Journal of Medical Investigation
ISSN
13431420
cat書誌ID
AA11166929
出版者
The University of Tokushima School of Medicine
44
1-2
開始ページ
47
終了ページ
51
並び順
47
発行日
1997-08
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系