ID 74628
著者
キムラ, カオリ Faculty of Dentistry, The University of Tokushima
ニシムラ, ユミコ Faculty of Dentistry, Okayama University
オヤマ, ケイスケ Faculty of Medicine, Saga University
カワナイ, タクヤ Faculty of Integrated Arts and Sciences, The University of Tokushima
ハシモト, エリカ Faculty of Integrated Arts and Sciences, The University of Tokushima
小山, 保夫 Faculty of Integrated Arts and Sciences, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
Tetracaine
Lidocaine
Zinc
Cytotoxicity
資料タイプ
紀要論文
抄録
In this study to examine the cytotoxic property of tetracaine, we cytometrically examined the effect of tetracaine on intracellular Zn2+ concentration by the use of FluoZin-3, a fluorescent indicator of intracellular Zn2+. Lidocaine was used as a reference drug. The incubation of rat thymocytes with tetracaine decreased the intensity of FluoZin-3 fluorescence while that with lidocaine increased the intensity. The incubation with 10 μM DTPA, a chelator for extracellular Zn2+, attenuated the tetracaine-induced decrease in fluorescence intensity. The application of ZnCl2 augmented FluoZin-3 fluorescence. The augmentation by ZnCl2 was a temperature-sensitive. Tetracaine attenuated the ZnCl2-induced augmentation of FluoZin-3 fluorescence. Taken together, the results suggest that tetracaine attenuates membrane Zn2+ influx, resulting in a decrease in intracellular Zn2+ concentration in rat thymocytes. Although the cells in this study are not targets for actions of local anesthetics, the result may give one clue to explain the difference between the cytotoxicity of local anesthetics since the action of tetracaine on FluoZin-3 fluorescence was opposite to that of lidocaine.
掲載誌名
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
cat書誌ID
AN10065859
25
2
開始ページ
7
終了ページ
13
並び順
7
発行日
2011
EDB ID
フルテキストファイル
言語
eng
部局
生物資源系