ID | 111902 |
Author |
Yamamoto, Jun
The University of Tokushima
Hirakawa, Hiroko
The University of Tokushima
Yamaguchi, Keiko
The University of Tokushima
Otaka, Akira
The University of Tokushima
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Keywords | FRET
Stimulus responsive
Peptide bond cleavage
UV irradiation
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Content Type |
Journal Article
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Description | In the field of chemical biology, methods for controlling peptidyl function by a stimulus are attracting increasing attention. Recently, we reported a stimulus-responsive peptide which can be cleaved after exposure to a stimulus. In this study, we developed a FRET-based assay system to estimate the kinetics of the stimulus-induced processing (peptide bond cleavage) reaction. Based on the FRET system, it was clarified that introduction of a sterically less-hindered or polar residue at the position adjacent to the stimulus-responsive amino acid accelerates the processing reaction.
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Journal Title |
Tetrahedron
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ISSN | 00404020
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NCID | AA00861787
AA11539786
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Publisher | Elsevier Ltd.
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Volume | 65
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Issue | 11
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Start Page | 2212
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End Page | 2216
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Published Date | 2009-01-20
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Rights | ©2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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EDB ID | |
DOI (Published Version) | |
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language |
eng
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TextVersion |
Author
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departments |
Pharmaceutical Sciences
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