ID 111902
著者
Yamamoto, Jun The University of Tokushima
Hirakawa, Hiroko The University of Tokushima
Yamaguchi, Keiko The University of Tokushima
キーワード
FRET
Stimulus responsive
Peptide bond cleavage
UV irradiation
資料タイプ
学術雑誌論文
抄録
In the field of chemical biology, methods for controlling peptidyl function by a stimulus are attracting increasing attention. Recently, we reported a stimulus-responsive peptide which can be cleaved after exposure to a stimulus. In this study, we developed a FRET-based assay system to estimate the kinetics of the stimulus-induced processing (peptide bond cleavage) reaction. Based on the FRET system, it was clarified that introduction of a sterically less-hindered or polar residue at the position adjacent to the stimulus-responsive amino acid accelerates the processing reaction.
掲載誌名
Tetrahedron
ISSN
00404020
cat書誌ID
AA00861787
AA11539786
出版者
Elsevier Ltd.
65
11
開始ページ
2212
終了ページ
2216
発行日
2009-01-20
権利情報
©2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
186631
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
薬学系