ID 111902
Author
Yamamoto, Jun The University of Tokushima
Hirakawa, Hiroko The University of Tokushima
Yamaguchi, Keiko The University of Tokushima
Keywords
FRET
Stimulus responsive
Peptide bond cleavage
UV irradiation
Content Type
Journal Article
Description
In the field of chemical biology, methods for controlling peptidyl function by a stimulus are attracting increasing attention. Recently, we reported a stimulus-responsive peptide which can be cleaved after exposure to a stimulus. In this study, we developed a FRET-based assay system to estimate the kinetics of the stimulus-induced processing (peptide bond cleavage) reaction. Based on the FRET system, it was clarified that introduction of a sterically less-hindered or polar residue at the position adjacent to the stimulus-responsive amino acid accelerates the processing reaction.
Journal Title
Tetrahedron
ISSN
00404020
NCID
AA00861787
AA11539786
Publisher
Elsevier Ltd.
Volume
65
Issue
11
Start Page
2212
End Page
2216
Published Date
2009-01-20
Rights
©2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
DOI (Published Version)
URL ( Publisher's Version )
FullText File
language
eng
TextVersion
Author
departments
Pharmaceutical Sciences