| ID | 111902 |
| Author |
Yamamoto, Jun
The University of Tokushima
Hirakawa, Hiroko
The University of Tokushima
Yamaguchi, Keiko
The University of Tokushima
Otaka, Akira
The University of Tokushima
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| Keywords | FRET
Stimulus responsive
Peptide bond cleavage
UV irradiation
|
| Content Type |
Journal Article
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| Description | In the field of chemical biology, methods for controlling peptidyl function by a stimulus are attracting increasing attention. Recently, we reported a stimulus-responsive peptide which can be cleaved after exposure to a stimulus. In this study, we developed a FRET-based assay system to estimate the kinetics of the stimulus-induced processing (peptide bond cleavage) reaction. Based on the FRET system, it was clarified that introduction of a sterically less-hindered or polar residue at the position adjacent to the stimulus-responsive amino acid accelerates the processing reaction.
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| Journal Title |
Tetrahedron
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| ISSN | 00404020
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| NCID | AA00861787
AA11539786
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| Publisher | Elsevier Ltd.
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| Volume | 65
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| Issue | 11
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| Start Page | 2212
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| End Page | 2216
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| Published Date | 2009-01-20
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| Rights | ©2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
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| EDB ID | |
| DOI (Published Version) | |
| URL ( Publisher's Version ) | |
| FullText File | |
| language |
eng
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| TextVersion |
Author
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| departments |
Pharmaceutical Sciences
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